研究業績一覧

・原著論文

38. Takahashi, S., Oshige, M., Katsura, S., Nagahara, Y. A new fluorescence labeling method for molecular analysis of double-stranded DNA. Anal. Biochem., Vol. 662, 115000, 2023.

37. Semba, Y., Yamamoto, S., Takahashi, S., Shinomiya, T., Nagahara, Y. c-kit inhibitor masitinib induces reactive oxygen species-dependent apoptosis in c-kit-negative HepG2 cells. Eur. J. Pharmacol., Vol. 931, 175183, 2022.

36. Takahashi, S., Shinomiya, T., Nagahara, Y. Azoxystrobin induces apoptosis and cell cycle arrest in human leukemia cells independent of p53 expression. Anticancer Res., Vol. 42, No. 3, 1307-1312, 2022.

35. Sakai, H., Shiina, I., Shinomiya, T., Nagahara, Y. BRAP2 inhibits the Ras/Raf/MEK and PI3K/Akt pathways in leukemia cells, thereby inducing apoptosis and inhibiting cell growth. Exp. Ther. Med., Vol. 21, No. 5, 463, 2021.

34. Zhang, Y., Yoshimi, Y., Funatsu O., Hayashi R., Komagawa S., Saito S., Nagahara, Y., Ikekita M. The mechanism of neurite outgrowth induction by novel synthetic retinobenzoic acids. bioRxiv, doi: https://doi.org/10.1101/2021.03.01.429320, 2021.

33. Fang, M., Shinomiya, T., Nagahara, Y. Cell death induction by Ranunculus ternatus extract is independent of mitochondria and dependent on Caspase-7. 3 Biotech, Vol. 10, No. 3, 123, 2020.

32. Iwasawa T., Shinomiya T., Ota N., Shibata N., Nakata K., Shiina I., Nagahara Y. Novel ridaifen-B structure analog induces apoptosis and autophagy depending on pyrrolidine side chain. Biol. Pharm. Bull., Vol. 42, No. 3, 401-410, 2019.

31. Toyama S., Migita O., Fujino M., Kunieda T., Kosuga M., Fukuhara Y., Nagahara Y., LI X.K., Okuyama T. Liver transplantation: New treatment for mucopolysaccharidosis type VI in rats. Pediatr. Int., Vol. 61, No. 2, 180-189, 2019.

30. Sikandan A., Shinomiya T., Nagahara Y. Ashwagandha root extract exerts anti‑inflammatory effects in HaCaT cells by inhibiting the MAPK/NF‑κB pathways and by regulating cytokines. Int. J. Mol. Med., Vol. 42, No. 1, 425-434, 2018.

29. Nagahara Y., Kawakami K., Sikandan A., Yagi D., Nishikawa R., Shinomiya T. Sphingoid base-upregulated caspase-14 expression involves MAPK. Biol. Pharm. Bull., Vol. 41, No. 5, 743-748, 2018.

28. Nagahara Y., Nagahara K. Relationship between structure and antiproliferative activity of novel 5-amino-4-cyanopyrazole-1-formaldehydehydrazono derivatives on HL-60RG human leukemia cells. Anticancer Res. Vol. 37, No. 11, 6329-6333, 2017.

27. Suita H., Shinomiya T., Nagahara Y. Caspase-6 induces 7A6 antigen localization to mitochondria during FAS-induced apoptosis of Jurkat cells. Anticancer Res. Vol. 37, No. 4, 1697-1704, 2017.

26. Nishio Y., Fujino M., Cai S., Kitajima Y., Saito T., Tsumura H., Ito M., Ito Y., Nagahara Y., Li X.K.. Impaired CD98 signaling protects against graft-versus-host disease by increasing regulatory T cells. Transpl. Immunol., Vol. 35, 34-39, 2016.

25. Okada T., Kimura A., Kanki K., Nakatani S., Nagahara Y., Hiraga M., Watanabe Y. Liver resident macrophages (Kupffer cells) share several functional antigens in common with endothelial cells. Scand. J. Immunol., Vol. 83, No. 2, 139-150, 2016.

24. Nagahara Y., Sekine H., Otaki M., Hayashi M., Murase N. Use of high concentrations of dimethyl sulfoxide for cryopreservation of HepG2 cells adhered to glass and polydimethylsiloxane matrices. Cryobiology, Vol. 72, No. 1, 53-59, 2016.

23. Nagahara Y., Nagahara K. N-(2-Amino-5-chlorobenzoyl) benzamidoxime derivatives inhibit human leukemia cell growth. Anticancer Res., Vol. 34, No. 11, 6521-6526, 2014.

22. Nishio Y., Fujino M., Zhao M., Ishii T., Ishizuka M., Ito H., Takahashi K., Abe F., Nakajima M., Tanaka T., Taketani S., Nagahara Y., Li X.K. 5-Aminolevulinic acid combined with ferrous iron enhances the expression of heme oxygenase-1. Int. Immunopharmacol., Vol. 19, No. 2, 300-307, 2014.

21. Nagahara Y., Morita M., Nakata T., Iba A., Shinomiya T. Loss of Bcl-2 expression correlates with increasing sensitivity to apoptosis in differentiating ES cells. Cell Biol. Int., Vol. 38, No. 3, 381-387, 2014.

20. Tamegai H., Takada Y., Okabe M., Asada Y., Kusano K., Katagiri Y.U., Nagahara Y. Aureobasidium pullulans culture supernatant significantly stimulates R-848-activated phagocytosis of PMA-induced THP-1 macrophages. Immunopharmacol. Immunotoxicol., Vol. 35, No. 4, 455-461, 2013.

19. Liu C., Zhu P., Saito T., Isaka Y., Nagahara Y., Zhuang J., Li X.K., Non-myeloablative conditioning is sufficient to induce mixed chimerism and subsequent acceptance of donor specific cardiac and skin grafts. Int. Immunopharmacol., Vol. 16, No. 3, 392-398, 2013.

18. Nagahara Y., Takeyoshi M., Sakemoto S., Shiina I., Nakata K., Fujimori K., Wang Y., Umeda E., Watanabe C., Uetake S., Yamori T., Dan S., Yoshimi Y., Shinomiya T., Ikekita M. Novel tamoxifen derivative ridaifen-B induces Bcl-2 independent autophagy without estrogen receptor involvement. Biochem. Biophys. Res. Comm., Vol. 435, No. 4, 657-663, 2013.

17. Nagahara Y., Suzuki E., Sekine Y., Uchiro H., Yoshimi Y., Shinomiya T., Ikekita M. SUTAF, a novel β-methoxyacrylate derivative, promotes neurite outgrowth with extracellular signal-regulated kinase and c-jun N-terminal kinase activation. Eur. J. Pharmacol., Vol. 694, No. 1-3, 53-59, 2012.

16. Liu Z., Hatayama N., Xie L., Kato K., Zhu P., Ochiya T., Nagahara Y., Hu X., Li X.K., Eicosapentenoic acid attenuates allograft rejection in an HLA-B27/EGFP transgenic rat cardiac transplantation model. Cell Med., Vol. 3, No. 1-3, 63-74, 2012.

15. Nagahara Y., Nagamori T., Tamegai H., Hitokuwada M., Yoshimi Y., Ikekita M., Shinomiya T. Inulin stimulates phagocytosis of PMA-treated THP-1 macrophages by involvement of PI3-kinases and MAP kinases. Biofactors, Vol. 37, No. 6, 447-454, 2011.

14. Fukui M., Nagahara Y., Nishio Y., Honjo T., Shinomiya T. Rokitamycin induces a mitochondrial defect and caspase-dependent apoptosis in human T-cell leukemia Jurkat cells. J. Pharmacol. Sci., Vol. 110, No. 1, 69-77, 2009.

13. Toyoda M., Yoshimi Y., Nagahara Y., Ikekita M. Lectin activity of major isoform of glucosidase II recognized Man8-9GlcNAc2. Res. Comm. Biochem. and Cell & Mol. Biol., Vol. 12, No. 1-2, 19-36, 2008.

12. Shiina I., Sano Y., Nakata K., Kikuchi T., Sasaki A., Ikekita M., Nagahara Y., Hasome Y., Yamori T., Yamazaki K. Synthesis and pharmacological evaluation of the novel pseudo-symmetrical tamoxifen derivatives as anti-tumor agents. Biochem. Pharmacol., Vol. 75, No. 5, 1014-1026, 2008.

11. Nagahara Y., Shiina I., Nakata K., Sasaki A., Miyamoto T., Ikekita M. Induction of mitochondria-involved apoptosis in estrogen receptor negative cells by a novel tamoxifen derivative, ridaifen-B. Cancer Sci., Vol. 99, No. 3, 608-614, 2008.

10. Shiina I., Sano Y., Nakata K., Suzuki M., Yokoyama T., Sasaki A., Orikasa T., Miyamoto T., Ikekita M., Nagahara Y., Hasome Y. An expeditious synthesis of tamoxifen, a representative SERM (selective estrogen receptor modulator), via the three-component coupling reaction among aromatic aldehyde, cinnamyltrimethylsilane and β-chlorophenetole. Bioorg. Med. Chem., Vol. 15, No. 24, 7599-7617, 2007.

9. Nagahara Y., Tanaka M., Shinomiya T. Mechanism of mitochondrial 7A6 antigen exposure triggered by distinct apoptotic pathways: involvement of caspases. Cytometry, Part A, Vol. 71A, No. 4, 232-241, 2007.

8. Kuramochi K., Matsui R., Matsubara Y., Nakai J., Sunoki T., Arai S., Nagata S., Nagahara Y., Mizushina Y., Ikekita M., Kobayashi S. Apoptosis-inducing effect of epolactaene derivatives on BALL-1 cells. Bioorg. Med. Chem. Vol. 14, No. 7, 2151-2161, 2006.

7. Esaka Y., Nagahara Y., Hasome Y., Nishio R., Ikekita M. Coenzyme Q2 induced p53-dependent apoptosis. Biochim. Biophys. Acta Vol. 1724, No. 1-2, 49-58, 2005.

6. Nagahara Y., Shinomiya T., Kuroda S., Kaneko N., Nishio R., Ikekita M. Phytosphingosine induced mitochondria-involved apoptosis. Cancer Sci. Vol. 96, No. 2, 83-92, 2005.

5. Matsuoka Y., Nagahara Y., Ikekita M., Shinomiya T. A novel immunosuppressive agent FTY720 induced Akt dephosphorylation in leukemia cells. Br. J. Pharmacol., Vol. 138, No. 7, 1303-1312, 2003.

4. Nagahara Y., Ikekita M., Shinomiya T. T cell selective apoptosis by a novel immunosuppressant, FTY720, is closely regulated with Bcl-2. Br. J. Pharmacol., Vol. 137, No. 7, 953-962, 2002.

3. Nagahara Y., Matsuoka Y., Saito K., Ikekita M., Higuchi S., Shinomiya T. Coordinate involvement of cell cycle arrest and apoptosis strengthen the effect of FTY720. Jpn. J. Cancer Res., Vol. 92, No. 6, 680-687, 2001.

2. Nagahara Y., Ikekita M., Shinomiya T. Immunosuppressant FTY720 induces apoptosis by direct induction of permeability transition and release of cytochrome c from mitochondria. J. Immunol., Vol. 165, No. 6, 3250-3259, 2000.

1. Nagahara Y., Enosawa S., Ikekita M., Suzuki S., Shinomiya T. Evidence that FTY720 induces T cell apoptosis in vivo. Immunopharmacology, Vol. 48, No. 1, 75-85, 2000.

・総説

1. Klionsky D.J., Nagahara Y, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, Vol. 12, No. 1, 1-222, 2016.

・国際会議論文

3. Nagahara Y., Uchida N., Midorikawa T., Murase N., Shinomiya T. Loss of mitochondrial function and accumulation of acidic vesicular organelles under low- temperature incubation in HaCaT cells. Proceedings of ICR2019, 2831-2836, 2019.

2. Sekine H., Otaki M., Hayashi M., Murase N., Nagahara Y. Estimation of DMSO concentration for cell cryopreservation on adhesion state. Proceedings of ICR2015, 503, 2015.

1. Yazawa T., Sekine H., Murase N., Nagahara Y. Mitochondrial reactive oxygen species involved in cold stress in HaCaT cells. Proceedings of ICR2015, 473, 2015.

・研究報告

4. 緑川 知輝、村勢 則郎、長原 礼宗. 低温処理による哺乳細胞へのオートファジー誘導. 低温生物工学会誌, Vol. 62, No. 2, 119-122, 2016.

3. 矢澤 智輝、関根 宏章、村勢 則郎、長原 礼宗. 低温ストレスによる細胞内シグナル経路の解明. 低温生物工学会誌, Vol. 60, No. 2, 109-113, 2014.

2. 関根 宏章，村勢 則郎，長原 礼宗. PDMS上に接着させた細胞の凍結保存におけるDimethyl sulfoxide濃度の検討. 低温生物工学会誌, Vol. 60, No. 2, 105-108, 2014.

1. 関根 宏章,村勢 則郎,長原 礼宗. 細胞接着状態での凍結保存に適したDimethyl sulfoxide濃度の検討. 低温生物工学会誌, Vol. 59, No. 2, 133-136, 2013.